The levels of key nucleolar proteins in the lymphoid cells of healthy individuals and patients with lymphoproliferative diseases
Malysheva, M.V.; Bulycheva, T.I.; Deĭneko, N.L.; Samoĭlova, R.S.; Kaplanskaia, I.B.; Magoulas, C.; Zatsepina, O.V.
Klinicheskaia Laboratornaia Diagnostika 12: 23-27
2010
ISSN/ISBN: 0869-2084 PMID: 21395050 Document Number: 646369
Immunocytochemical staining with specific antibodies was used to study the expression of three nucleolar proteins (fibrillarin, B23/nucleofozmin, and SURF6), which were involved in pRNA maturation, in the lymphoid cells of healthy individuals and patients with lymphoproliferative diseases and to compare it with the expression of the known proliferation marker Ki-67 protein. The results indicated that fibrillarin was detectable at the comparable level in the lymphoid cells of the patients and in the peripheral blood lymphocytes of the healthy individuals. In one fourth of the patients, the proportion of cells containing B23/nucleofozmin was noticeably higher than that in the lymphocytes of donors; however, there was no great difference in patients with different types of the disease. The number of SURF6-positive cells was directly correlated with that of Ki-67-positive cells. The maximum level (47-67%) of SUR6-positive lymphoid cells was found in splenic lymphosarcomas and mantle cell lymphoma. The findings suggest that SURG6 protein may be of additional diagnostic and prognostic value.