Determination of GATA-4 in the testis of the mouse
Yang, J.-Z.; Zhao, Y.-F.; Wang, Y.-M.; Jing, C.-X.; Miao, N.-Z.; Ai, Q.-Y.
Zhonghua Nan Ke Xue 16(10): 901-904
2010
ISSN/ISBN: 1009-3591 PMID: 21243753 Document Number: 646186
To explore the characteristics and distribution of GATA-4 in the testis of male mice. Paraffin sections were obtained from the testes of 24 male B6SJLF1/J mice, aged 0 day (n = 6), 2 weeks (n = 6), 4 weeks (n = 6) and 6 weeks (n = 6), and the expressions of GATA-4 in the testis were observed by the immunohistochemical ABC method and DAB visualization at different times. Positive expressions of GATA4 were found in the Sertoli cells and Leydig cells of all the mice, but significantly higher in the 4- and 6-week-old than in the 0-day and 2-week-old groups (P < 0.01). And they were also observed in the germ cells of the 4- and 6-week-old mice, significantly higher in the latter than in the former (P < 0.01). GATA-4 exists in the testis of male mice, which has provided a morphological base for sex determination and differentiation and hormone regulation in the testis.