Apoptosis effects on human esophageal cancer cells by soyasaponin Bb and its machanism
Pei, Y.; Zhao, H.; Du, X.; Li, J.
Wei Sheng Yan Jiu 39(4): 444-446
2010
ISSN/ISBN: 1000-8020 PMID: 20726234 Document Number: 645592
To explore the effect and potential mechanism of apoptosis in the Eca-9706 cells induced by soyasaponin Bb. Different concentrations of soyasaponin Bb were on the Eca-9706 cell. Cell growth suppression rate was detected by MTT. Apoptotic rate was detected by TUNEL. The expression changes of HDAC1, NF-kappaB, PTEN, cyclin D1, c-met, VEGF or caspase-3 in the SSBb were detected by immunohistochemistry and immunoblotting. Compared with the control group, the growth-inhibition rate and apoptotic rate were increased, the expressions of PTEN, caspase 3 were increased, the expressions of c-met, VEGF, HDAC1, NF-kappaB and cyclin D1 were decreased. SSBb can suppress Eca-9706 cell growth. SSBb can exhibit reverse effects on over expression of c-met, VEGF in Eca-9706 cells. Eca-9706 cell apoptosis can be induced by SSBb through inhibiting HDAC1-NF-kappaB and activating PETEN and caspase-3 signaling pathways.