Use of quantitative fluorescence polymerase chain reaction in the invasive prenatal diagnosis of Down's syndrome
Kriventsova, N.V.; Shokarev, R.A.; Avrutskaia, V.V.; Kriger, S.I.; Klochkova, N.E.; Gimbut, V.S.; Kornienko, I.V.
Klinicheskaia Laboratornaia Diagnostika 8: 27-30
2010
ISSN/ISBN: 0869-2084 PMID: 20891040 Document Number: 643643
A quantitative fluorescence polymerase chain reaction (QF-PCR) technique based on the determination of triple-dose chromosome-specific short tandem repeats (STR) has been recently developed for the prenatal diagnosis of numeral abnormalities of chromosomes 21, 18, 13 and X and Y. This investigation examined 55 blood samples from healthy donors, 17 amniotic fluid samples, 27 blood samples from children with regular trisomy 21, 1 sample with a translocation variant of Down's syndrome, and 3 samples with triploidy. The heterozygosity of 4 STR markers specific for chromosome 21 was determined, which were used in QF-PCR to detect Down's syndrome.