Comparison of Monoclonal and Polyclonal anti-P 1 Reagents
Strobel, E.
Clinical Laboratory 47(5-6): 249-255
2001
ISSN/ISBN: 1433-6510 PMID: 11405603 Document Number: 6413
Whereas monoclonal reagents for ABO, Rhesus, Kell, Kidd, Lewis and MNS antigens are widely used in blood grouping laboratories and have been well evaluated, little attention has been given so far to monoclonal anti-P 1 reagents. Therefore it was the aim of our study to examine the suitability of monoclonal anti-P 1 reagents for routine use in comparison to polyclonal anti-P 1 reagents. 10 polyclonal anti-P 1 reagents (9 from goat, 1 from rabbit) and 5 monoclonal anti-P 1 reagents (4 with clone 650, 1 with clone OSK17) were tested by the tube-spinmethod at room temperature (incubation time as prescribed by the manufacturer) using untreated P 1 + red blood cells (RBCs) for titration and untreated P 1 - RBCs as negative controls. P 1 - RBCs with a positive direct antiglobulin test (DAT+) (coated with incomplete anti-D) and sialidase-treated P 1 - RBCs (T+) were used to recognize false positive reactions. 3 monoclonal anti-P 1 reagents had a titer of ≤ 1 with P 1 + weak RBCs, but alt polyclonal anti-P 1 reagents showed a titer ≥ 2. Negative controls (untreated P 1 - RBCs) were inconspicuous. 7 polyclonal and 1 monoclonal anti-P 1 reagents showed false positive reactions with P 1 - DAT+ RBCs. All polyclonal and 1 monoclonal (containing clone OSK17) reagent showed false positive reactions with P 1 - T+ RBCs. As several monoclonal anti-P 1 reagents are less suitable for routine use than polyclonal anti-P 1 reagents, quality control of anti-P 1 reagents should be intensified.
Document emailed within 1 workday
