Isl-1 down-regulates DRG cell proliferation during chicken embryo development
Chen, D.; Wang, G.; Luo, H.; Liu, J.; Cui, S.
Neuro Endocrinology Letters 31(1): 67-72
2010
ISSN/ISBN: 0172-780X PMID: 20150874 Document Number: 639016
Protein Isl-1 RNA interference and over expression in early chicken embryo dorsal root ganglia (DRG) were used to investigate the function of Isl-1 in DRG cell proliferation. Isl-1 targeted shRNA expression vector and Isl-1 over-expression vector were transfected into chicken embryo DRG by in ovo electroporation. Then, the DRG proliferation rate was detected by BrdU immunohistochemistry. The rate of DRG cell proliferation increased after Isl-1 knock-down and decreased after Isl-1 over-expression. In this study, we found that Isl-1 negatively modulates DRG cell proliferation.