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Study of immunogenicity of chlorogenic acid-BSA with different number of CGA

Yuan, L.; Liu, P.; Hu, Y.; Liao, H.; Mu, L.; Lin, H.; Huang, H.

Zhongguo Zhong Yao Za Zhi 35(20): 2756-2759

2010

ISSN/ISBN: 1001-5302
PMID: 21246835
Document Number: 638698
To investigate the immunogenicity of chlorogenic acid-BSA(CGA-BSA) and the foundation for the allergization of CGA-BSA. The CGA-BSA with different number of CGA was synthesized to allergize the BALB/c mice and SD rats to get antiserum. The level of IgE and histamine was analyzed by ELISA, and the passive cutaneous anaphylaxis (PCA) test was carried to analyze the antibody titer. When the coupling rate of CGA-BSA is 20, the highest IgE and histamine level in BALB/c mice and the antibody titer in PCA are the highest. It has better immunogenicity when the coupling rate of CGA-BSA is between 10 to 20, while the CGA-BSA 20 with the best immunogenicity.

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