Intestinal lamina propria TcRgammadelta+ lymphocytes selectively express IL-10 and IL-17
Asigbetse, K.E.; Eigenmann, P.A.; Frossard, C.P.
Journal of Investigational Allergology and Clinical Immunology 20(5): 391-401
2010
ISSN/ISBN: 1018-9068 PMID: 20945605 Document Number: 638073
The characteristics and roles of gut lymphocytes have been only partly elucidated, in particular with regard to activation patterns. To characterize lymphocytes from various parts of the gut and examine their activation pattern as a network. Lymphocytes were isolated from the epithelium, the lamina propria, Peyer's patches, mesenteric lymph nodes, the spleen, and peripheral blood of naive mice. They were then characterized for T cell phenotype, T cell receptors (TcRs), activation markers, and cytokine production. The results showed a gradient of cells with an increasing proportion of TcRgammadelta+, CD8alphaalpha+ cells towards the gut lumen, with the highest number found in intraepithelial lymphocytes. These cells, together with lamina propria lymphocytes (LPLs) were also characterized by a memory-like phenotype (CD25- CD45RB(low) and CD44(high)) and CD69 expression. CD8+ TcRgammadelta+ LPLs produced IL-10 and IL-17, while TcRalphabeta+ LPLs were FoxP3 positive. Gut lymphocytes express various receptors and cytokines according to their location. These specific features suggest a differential function for gut lymphocytes depending on their location.