Experimental study on in vitro lamina propria engineering using oral fibroblast and polyglycolic acids
Zhou, Z.-t.; Huang, H.; Li, Q.; Cao, D.-j.; Liu, W.; Cao, Y.-l.
Shanghai Kou Qiang Yi Xue 13(1): 30-33
2004
ISSN/ISBN: 1006-7248 PMID: 15007477 Document Number: 583454
This study investigated the feasibility of lamina propria engineering in vitro using expanded oral fibroblast(OFC) and Polyglycolic Acids (PGA). OFC were isolated by tissue explant method and expanded in vitro. OFC (20x10(6)) of 3rd passage were collected and then seeded onto PGA unwoven fibers to form a cell-scaffold. The constructs were cultured in DMEM +10% FBS. The cell-scaffold constructs were observed continuously by microscope. Small fragments were harvested at 1 week for electromicroscope, histological and RT-PCR analysis. At the sixth day, a neo-lamina propria was formed. HE and Masson stain revealed the formation of collagen fibers. RT-PCR revealed the new forming collagen was mainly type I collagen. lamina propria tissue is possible to engineer in vitro using oral fibroblast and polyglycolic acids. At this basis, we can construct bi-layer tissue engineering oral mucosa in the further research.