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Survivin may enhance DNA double-strand break repair capability by up-regulating Ku70 in human KB cells

Jiang, G.; Ren, B.; Xu, L.; Song, S.; Zhu, C.; Ye, F.

Anticancer Research 29(1): 223-228

2009

ISSN/ISBN: 0250-7005
PMID: 19331153
Document Number: 636599
Survivin, expressed in almost all types of human malignancies, functions as a key factor in radioresistance primarily by inhibiting apoptosis. This study was conducted to investigate whether survivin plays a role in the DNA repair process in the KB human squamous cell carcinoma cell line. A stable KB cell line overexpressing survivin was established through the use of pIRES2-EGFP vector containing the coding region of survivin. Cells were then irradiated with X-rays and evaluated for DNA double-strand breaks (DSBs) by comet assay and flow cytometry for phospho-histone gammaH2AX. The protein levels of some DSB repair genes were detected by Western blotting analysis. Comet assay and flow cytometry for phospho-histone gammaH2AX showed that overexpression of survivin resulted in significantly fewer DSBs in irradiated cells. Among the DSB repair genes detected, the protein level of Ku70 was up-regulated in survivin-overexpressing KB cells. This finding suggests that survivin may enhance DSB repair capability in KB cells by up-regulating Ku70.

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