An experimental luminescent immunochromatographic system
Iarkov, S.P.; Skopinskaia, S.N.; Shilenko, I.V.; Zlobin, V.N.
Vestnik Rossiiskoi Akademii Meditsinskikh Nauk 3: 20-26
2009
ISSN/ISBN: 0869-6047 PMID: 19385426 Document Number: 636498
Luminescent immunochromatographic assay for antigens of microorganisms was developed to detect antigens and cells of microorganisms, toxins. Luminescent submicrone latex particles, quantum labels, ganglioside-containing liposomes with sulforhodamine-01 in the internal volume were used as a luminescent mobile phase for conjugation with antibodies. Devices for registration of luminescent immunochromatograms were developed. Submicrone latexes were shown to have advantages over quantum labels, ganglioside-containing liposomes with sulforhodamine-101 and colloid gold for immunochromatographic procedures. Sensitivity of luminescent latex-based immunochromatographic indicator element was 3-30 that elements based on colloid gold conjugates for identical microorganisms and elements.