Development of a suspension array method for quantitative detection of Y. pestis
Wang, J.; Shan, L.; Yang, Y.; Sun, X.; Yang, Y.; Hu, K.; Hou, Y.; Ding, Y.
Wei Sheng Yan Jiu 38(5): 607-611
2009
ISSN/ISBN: 1000-8020 PMID: 19877528 Document Number: 636118
To establish a platform for rapid, sensitive, specific, high-throughput, and simultaneous detection of multiple biothreat-associated agents, a suspension array-based immunoassay was developed for exploring the feasibility of directly simultaneous detection of Y. pestis in powder samples. The immunoassay using coupled fluorescent beads were employed to detect Y. pestis as a model from powder sample and the feasibility for detection in field samples was demonstrated by both blind and standard laboratory trials. The newly developed suspension array appeared to be specific and sensitive, with the detection sensitivities of 0.154 ng/ml for Y. pestis F1 antigen, and the dynamic ranges of 0.154 - 4514 ng/ml, which were higher than those of corresponding conventional ELISA tests. The suspension array could rapidly, sensitively, specifically and quantitatively detect pathogen from powder samples, which would be useful for early identification, rapid diagnosis, and response for the attack of bioterrorism and outbreak of infectious disease.