Determination of plasma protein binding rate of bufalin
Liu, Y.; Chen, Z.; Tao, B.; Feng, N.; Zhao, J.
Zhongguo Zhong Yao Za Zhi 34(21): 2817-2820
2009
ISSN/ISBN: 1001-5302 PMID: 20209923 Document Number: 632938
To study plasma protein binding rate of bufalin. HPLC was employed to determine the concentration of bufalin. Plasma protein binding rate studies were conducted by equilibrium dialysis method. The influence of drug concentration and plasma in different species on plasma protein binding rate were studied. There was no significant difference in the plasma protein binding rates at low, middle and high bufalin concentrations in dilution medium. The protein binding rate of bufalin in human plasma was higher than in the rat plasma. Bufalin has higher protein binding extent with both rat plasma and human plasma.