Investigation of ion channel mechanism for the regulatory volume decrease in lung adenocarcinoma cells
He, W.; Li, H.; Liu, J.; Shi, L.-p.; Zhang, Z.-f.; Xu, M.; Wang, J.
Zhonghua Yi Xue Za Zhi 89(45): 3220-3223
2009
ISSN/ISBN: 0376-2491 PMID: 20193538 Document Number: 632454
This study was designed to observe the regulatory volume decrease (RVD) process in human lung adenocarcinoma cells (A549) and to investigate its ion channel mechanism. Electric measurement system of cell volume was used to detect the cell volume changes following exposure to hypotonic solution. Whole-cell patch clamp recordings were applied to investigate the characteristics of the volume-sensitive Cl(-) channel in A549 cells. Extracellular hypotonicity induced cell swelling followed by a typical RVD process, which can be inhibited by Cl(-) channel blocker (NPPB 100 micromol/L) and K(+) channel blocker (CsCl 5 mmol/L). Meanwhile, a outward-rectifying chloride currents which was sensitive to NPPB and DIDs was recorded in A549 using the whole cell patch clamp. The human lung adenocarcinoma cells has RVD process which is dependent on the parallel activation of Cl(-) channel and K(+) channel. The volume-sensitive Cl(-) channel is involved in volume regulation of lung adenocarcinoma cells.