Effects of PCB153 on oxidative stress and 8-OHdG content induced by PBDE-47 in human neuroblastoma cells in vitro
Gao, P.; He, P.; Wang, A.; Xia, T.; Xu, Z.; Niu, Q.; Guo, L.; Chen, X.
Wei Sheng Yan Jiu 38(5): 513-515
2009
ISSN/ISBN: 1000-8020 PMID: 19877502 Document Number: 631029
To explore the effects of PCB153 (2,2,4,4,5,5-hexachlorobiphenyl) on oxidative stress and 8-OHdG (8-hydroxy-2'-deoxyguanosine) content induced by PBDE-47 (2,2,4,4-tetrabromodiphenyl ether) in SH-SY5Y cells. SH-SY5Y cells were incubated with different concentrations of 1, 5, 10 micromol/L PBDE-47 or/and 5 micromol/L PCB153 and antioxidant N-acetyl cysteine (NAC 100 micromol/L) for 24h in vitro, ROS (reactive oxygen species) level and 8-OHdG were measured by using DCFH-DA fluorescent marking method and the skill of high performance liquid chromatogram-electrochemistry (HPLC-EC), respectively. As for ROS formation, there was no significant difference in PBDE-47 groups alone compared to the control group and their corresponding combined groups, respectively (P > 0.05). ROS formation were significantly increased in 5 and 10 micromol/L combined groups compared to the control group and their corresponding concentrations of PBDE-47 groups or PCB153 group, respectively (P < 0.05). 8-OHdG levels were significantly increased in 10 micromol/L PBDE-47, 5 micromol/L PBDE-47 + 5 micromol/L PCB153, and 10 micromol/L PBDE-47 + 5 micromol/L PCB153 groups compared with the control (P < 0.05). 8-OHdG level was dramatically increased in 10 micromol/L PBDE-47 + 5 micromol/L PCB153 groups compared to their corresponding doses of PBDE-47 groups or PCB153 group (P < 0.05). The groups coincubation with N-acetylcysteine caused a decrease in cellular ROS level and ameliorated PBDE-47-mediated oxidative DNA damage effects. Positive relationship was observed between ROS level and 8-OHdG contents (r = 0.895, P < 0.01). These results provide evidence of a direct or indirect relationship between PBDE-47 and oxidative DNA damage. Furthermore, PBDE-47 combined with PCB153 may increase the effects on oxidative DNA damage in SH-SY5Y cells in vitro, oxidative stress may responsible for DNA damage induced by PBDE-47.