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Expression profiles of apoptotic genes of pig peripheral blood leukocytes caused by classical swine fever virus infection

Shi, Z.-X.; Sun, J.-F.; Guo, H.-C.; Tu, C.-C.

Bing du Xue Bao 24(6): 456-463

2008

ISSN/ISBN: 1000-8721
PMID: 19226955
Document Number: 622872
Classical swine fever (CSF) is a contagious swine disease charactered by hemorrhagic fever and leukopenia,usually leading to substantial economic losses. To obtain a insight of leucopenia caused by CSFV infection, DNA microarray analyses of peripheral blood leucocytes (PBL) of the infected pigs was performed. Three health pigs were inoculated with a lethal dose of CSFV Shimen strain and their PBLs were isolated when the onset of typical clinical signs and then subjected to total RNA extraction followed by microarray analysis with Affymetrix Porcine Genome Array GeneChips. The results showed that the significant differences were observed in cellular apoptotic genes expression at 7 days post-infection (p. i.). The changes of the genes expression were confirmed by real time RT-PCR of some selected apoptosis-related genes. This study provided a valuable information for further investigating the molecular mechanism of apoptosis caused by CSFV infection.

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Futami, H.; Eader, L.A.; Komschlies, K.L.; Bull, R.; Gruys, M.E.; Ortaldo, J.R.; Young, H.A.; Wiltrout, R.H. 1991: Flavone acetic acid directly induces expression of cytokine genes in mouse splenic leukocytes but not in human peripheral blood leukocytes Cancer Research 51(24): 6596-6602
Iushchenko, I.A.; Kolontsov, A.A.; Shubina, N.G. 1998: Procoagulant activity in swine leukocytes, infected with the swine classical plague virus Voprosy Virusologii 43(3): 138-141
Hergarten, G.; Hurter, K.P.; Hess, R.G. 2001: Detection of infection with classical swine fever virus in wild boar: a comparison of different laboratory diagnostic methods Deutsche Tierärztliche Wochenschrift 108(2): 51-54
Parchariyanon, S.; Inui, K.; Damrongwatanapokin, S.; Pinyochon, W.; Lowings, P.; Paton, D. 2000: Sequence analysis of E2 glycoprotein genes of classical swine fever viruses: identification of a novel genogroup in Thailand Deutsche Tierärztliche Wochenschrift 107(6): 236-238
Kulesko, I.I.; Sobolev, N.M. 1973: Serological types of the classical swine fever virus Archiv für Experimentelle Veterinarmedizin 27(4): 661-666
Xu, Y.-Z.; Zhou, Y.-J.; Tong, W.; Li, L.; Jiang, Y.-F.; Tong, G.-Z. 2013: Study on using NSP2 protein of porcine reproductive and respiratory syndrome virus (HuN4-F112) to express E2 neutralizing epitope of classical swine fever virus Bing du Xue Bao 29(1): 17-25
Krivonos, A.V.; Zaberezhny, A.D.; Grebennikova, T.V.; Gibadulin, R.A.; Musienko, M.I.; Tsibezov, V.V.; Bogdanova, V.S.; Kal'nov, S.L.; Aliper, T.I.; Nepoklonov, E.A. 2000: Synthesis and immunochemical properties of the recombinant major surface glycoprotein E2 of the classical swine fever virus Voprosy Virusologii 45(2): 29-36
Xu, Q.; Zhang, X.; Jing, J.; Shi, B.; Wang, S.; Zhou, B.; Chen, P. 2015: Hsp70 Fused with the Envelope Glycoprotein E0 of Classical Swine Fever Virus Enhances Immune Responses in Balb/c Mice Bing du Xue Bao 31(4): 363-369
Markowska-Daniel, I.; Pejsak, Z.; Szmigielski, S.; Jeljaszewicz, J.; Pulverer, G. 1993: Prophylactic application of Propionibacterium avidum KP-40 in swine with acute experimental infections. I. Viral infections--Aujeszky's disease and classical swine fever Deutsche Tierärztliche Wochenschrift 100(4): 149-151
Paton, D.J.; Greiser-Wilke, I. 2003: Classical swine fever--an update Research in Veterinary Science 75(3): 169-178
Devare, S.G.; Desai, S.M.; Rupprecht, K.R.; Allen, R.G.; Dawson, G.J.; Hunt, J.C.; Casey, J.M. 1988: Genes of human immunodeficiency virus, type i (HIV-I), their expression in Escherichia coli, and their utility in diagnosis of virus infection Indian Journal of Biochemistry and Biophysics 25(6): 504-509
Paludan, Søren.R.; Melchjorsen, J.; Malmgaard, L.; Mogensen, Søren.C. 2002: Expression of genes for cytokines and cytokine-related functions in leukocytes infected with Herpes simplex virus: comparison between resistant and susceptible mouse strains European Cytokine Network 13(3): 306-316
Eskildsen, M.; Overby, E. 1976: Serological diagnosis of classical swine fever: a comparison of a modified direct complement fixation test with an immunofluorescence plaque neutralization test in the diagnosis of experimental subclinical infection Acta Veterinaria Scandinavica 17(2): 131-141
Wittmann, G.; Ahl, R.; Lorenz, R.J. 1987: Incidence and control of classical swine fever in West Germany Archiv für Experimentelle Veterinarmedizin 41(6): 825-827
Bellani, L.; Caporale, G. 1971: Diagnosis, clinical course and control measures of classical swine fever Bulletin - Office International des Epizooties 75(9): 611-625
Elbers, A.R.W.; Loeffen, W.L.A.; Koch, G. 2012: Classical swine fever and avian influenza epidemics: lessons learned Berliner und Munchener Tierarztliche Wochenschrift 125(1-2): 21-26
Blome, S.; Gabriel, C.; Beer, M. 2013: Possibilities and limitations in veterinary vaccine development using the example of classical swine fever Berliner und Munchener Tierarztliche Wochenschrift 126(11-12): 481-490
Deptuła, W.; Buczek, J. 1987: Phagocytic activity of peripheral blood leukocytes in bulls experimentally infected with IBR/IPV virus (bovid herpesvirus 1) Acta Microbiologica Polonica 36(4): 293-301
Stopchanskaia, A.G.; Novitskiĭ, V.I.; Oleĭnik, G.I.; Prilutskaia, N.I. 1978: Electron-microscopic study of the relationship between influenza a virus (H3N2) and human peripheral blood leukocytes Voprosy Virusologii 4: 422-427
Valentová, V.; Psikal, I.; Kovarcík, K. 2003: Demonstration of bovine respiratory syncytial virus RNA in peripheral blood leukocytes of naturally infected cattle Acta Virologica 47(1): 33-36