Study on stability of baicalin and baicalein in Rat plasma by HPLC-ECD
Wang, Q.; Zhang, Y.-j.; Li, W.-f.; Yang, J.; Jiang, H.; Sun, W.-j.
Zhongguo Zhong Yao Za Zhi 33(22): 2675-2678
2008
ISSN/ISBN: 1001-5302 PMID: 19216170 Document Number: 622822
To compare the stability of baicalin and baicalein in buffered aqueous solutions and mouse plasma in order to discuss the degradation mechanism and the method of disposing samples. HPLC-ECD was used to determine baicalin and baicalein in different conditions, and the disposing samples methods were selected to stabilize them. Baicalin and baicalein in the buffered aqueous solution at pH 7.4 was more stable than that in the mouse plasma. After adding Vit C in the buffered aqueous solution, the stability of baicalin and baicalein could be improved obviously and their remained percents were 102% and 100%. The stability of baicalin and baicalein could be enhanced distinctly in the mouse plasma only when adding Vit C and 1 mol x L(-1) HCl, and furthermore their remained percentage was up to 98% and 103%. The stability of baicalin and baicalein is related to the oxidation and enzyme degradation and it can be improved by adding Vit C and 1 mol x L(-1) HCl in the solution.