DNA typing of minimal oral epithelial cell samples
Yang, D.; Liu, C.; Xu, Q.-Y.; Hu, H.-Y.; Liu, H.
Fa Yi Xue Za Zhi 24(2): 126-128
2008
ISSN/ISBN: 1004-5619 PMID: 18605044 Document Number: 620965
To seek simple and cost-effective extraction technique to improve multiplex STR amplification from minimal oral epithelial cell samples. One hundred DNA samples of oral epithelial cells extracted by mini system Chelex-100 method were quantitated by ABI 7500 Real Time System and were then typed with Identifiler system in ABI 3130 Genetic Analyzer. The DNA contents of different categories of samples were as followings: suck pipes (0.72-116.78 ng), cup edges (2.15-142.5 ng), mouths of drink bottle (1-34.65 ng), chopsticks (3.35-26.6 ng), fruit cores (0.294-21.4 ng), and poultry bones (0.88-5.88 ng). The mean successful typing rate for gender and more than 9 STR loci was about 59.38%. Except the addition or no addition of proteinase K to the samples, all other factors-C users' variation, sample extraction methods, and qualities and properties of the samples had considerable effects on the contents of extracted DNA. Successful STR typing can be achieved in about 60% minimal oral epithelial cell DNA samples extracted by mini Chelex system.