The role of expired air moisture condensate in assessment of pulmonary inflammation in patients with chronic obstructive pulmonary disease
Dotsenko, E.K.; Goncharova, V.A.; Kuzubova, N.A.; Kamenova, M.Iu.; Egorova, N.V.
Terapevticheskii Arkhiv 80(3): 10-14
2008
ISSN/ISBN: 0040-3660 PMID: 18441676 Document Number: 620601
Aim. To study biochemical composition of expired air condensate (EAC) in patients with chronic obstructive pulmonary disease (COPD) in relation to a phase and severity of the disease and its treatment.Material and methods. EAC was investigated in 18 COPD patients and 9 healthy subjects. Basic broncholytic therapy with ipratropium bromide was combined with beclomethasone and Fenspiride in 11 and 7 patients, respectively. The condensate was 1yophilised, the residue was solved and analysed on the biochemical analyzer Casis (Beringer Manheim, Rosch). E4 C was examined for albumin, Creactive protein, glucose, cholesterol, triglycerides, urea, uric acid, alkaline phosphatase (AP), lactate dehydrogenase, gamma-glutamyl transferase, total calcium, magnesium.Results. Compared to healthy subjects, COPD patients' EA C contains significantly higher levels of albumin, C-reactive protein, calcium, bilirubin and more active AP. Quantitative composition of EAC depends on COPD phase and severity. A negative correlation exists between FEV+AEA-1 and albumin concentration, FEV+AEA-1 and CKP concentration. The anti-inflammatory therapy decreases EA C content of both protein and lipid metabolism products, enzyme activity reflecting attenuation of oxidant and inflammatory processes, stabilization of cell membranes in the respiratory zone.Conclusion. EA C composition reflects metabolic processes in the lungs and can be used for assessment of airway affection, activity of the inflammatory process and COPD treatment efficacy.