Different change of olive tail moment and cytokinesis-block micronucleus frequency of the cells of 4 kinds treated with polycyclic aromatic hydrocarbon
Cheng, J.; Leng, S.; Niu, Y.; Duan, H.
Wei Sheng Yan Jiu 37(3): 273-276
2008
ISSN/ISBN: 1000-8020 PMID: 18646520 Document Number: 620185
To investigate the difference change of olive tail moment (TM) and cytokinesis-block micronucleus (CBMN) frequency of the cells of four kinds treated with polycyclic aromatic hydrocarbon. Four different cell lines, CHO-K1, NIH3T3, CHL, and HELF were treated with B(a)P in vitro, and the genetic damage were detected by Comet and CBMN assay, respectively. The positive controls were Hydro peroxides (H2O2 ) and Cyclohosphamide (CP) for Comet and CBMN assay, respectively. The changes of olive TM levels of four cell lines treated with B(a)P were occurred at the lower doses of B(a)P (CHO - K1:0.5 micromol/L, NIH3T3 : 0.75 micromol/L, CHL : 0.5 micromol/L, HELF : 0.3125 micromol/L) than that of CBMN frequencies (CHO-K1 : 2.0 micromol/L, NIH3T3 : 1.51 micromol/L, CHL : 2.0 micromol/L, HELF : 1.25 micromol/L). The dose-response curve of B(a)P was linear for Olive TM, but sigmoid for CBMN frequency. The data showed that olive TM was more sensitive than CBMN frequency for the genotoxic detects of B(a)P for the four cell lines.