Mechanisms of interaction of tetrahydrocortisol and its complex with apolipoprotein A-I and DNA regulatory cis-elements of CC (GCC) 5/GG (CGG) 5 type
Panin, L.E.; Kunitsyn, V.G.; Poliakov, L.M.
Biofizika 53(1): 42-47
2008
ISSN/ISBN: 0006-3029 PMID: 18488499 Document Number: 620168
An IR-spectroscopy study of the mechanism of interaction between duplex CC(GCC)(5)/GG(CGG)(5)Li-2 and tetrahydrocortisol or tetrahydrocortisol-apolipoprotein A-I complex revealed the formation of hydrogen bonds between the OH group of the tetrahydrocortisol A-ring and the C=0 group of cytosine or guanine. Tetrahydrocortisol forms hydrogen bonds with the PO2-group of the duplex and with the OH-group of monosaccharide. The interaction of tetrahydrocortisol and apolipoprotein A-I with the duplex occurs at the same active site, namely, with the C = O-group of bases. The order --> order structural transition takes place in the duplex under the action of tetrahydrocortisol. The order --> disorder structural transition takes place in the duplex under the action of tetrahydrocortisol-apolipoprotein A-I complex.