The effect of continuously compressive press on the shape of cells and expressions of MMP-9,TRAP in human osteoclasts
Xu, H-Yan.; Zhou, H.; Rao, G-Zhou.
Shanghai Kou Qiang Yi Xue 17(3): 285-288
2008
ISSN/ISBN: 1006-7248 PMID: 18661071 Document Number: 619111
To investigate the effect of continuously compressive pressure on the function of human osteoclasts. Monocytes were collected from umbilical cord cells, and were cultured for 14 days. alpha-MEM which contains RANKL and M-CSF was used as the culture medium. 100kPa compressive pressure was applied to the mature osteoclasts for 1 hour,2 hours and 3 hours. The shape change was observed. TRAP,MMP-9 enzymes were stained, and measured randomly. The data were analyzed with SPSS12.0 software package for Student's t test. Compressive pressure made the shape of osteoclasts incline to round. After adding pressure for 1 hour, there was significant difference on the expression of TRAP between pressure group(59.61+/-14.95)and non-pressure group(80.01+/-9.69) (P<0.05). Significant increase expression of MMP-9 occured after 5 hours (P<0.05). The changes of shape and levels of functional enzymes show that compressive pressure enhances the bone-absorbing activity. Supported by National Natural Science Foundation of China (Grant No.30471911).