Human cord blood-derived large myeloid cells: spontaneous shape change and chemotactic movement
Roberts, G.M.; Laffafian, I.
British Journal of Biomedical Science 53(2): 96-100
1996
ISSN/ISBN: 0967-4845 PMID: 8757685 Document Number: 459353
We have demonstrated two aspects of the movement of large myeloid cells derived from cord blood mononuclear cells after culture in RPMI 1640 and 10% fetal calf serum for eight days. At this time cells assume various shapes including round, oval, veiled, elongated and dendritic. Interestingly, these shapes were not static. Time-lapse recording demonstrated that individual cells change shape quite dramatically over four hours. Some of the veils showed a great deal of activity, some elongated cells shrank into rounded forms, and rounded cells stretched out into dendritic cells or elongated forms. We have previously shown that the number of N-formyl-methionine-leucyl-phenylalanine receptors on the cell surface increase with culture time up to eight days. Furthermore, a rise in Ca-2+ was observed in response to 1 mu-mol/L N-formyl-methionine-leucyl-phenylalanine (chemotactic peptide) at the single cell level. In this paper we show the motility of cord blood-derived myeloid cells towards a chemotactic peptide concentration gradient leaking from a micropipette.