Application of polymerase chain reaction with confronting two-pair primers in aldehyde dehydrogenase-2 typing
Ye, X-ping.; Peng, T.; Xiao, K-yin.; Su, Z-xiong.; Shang, L-ming.; Li, L-qun.
Zhonghua Yu Fang Yi Xue Za Zhi 42(2): 119-122
2008
ISSN/ISBN: 0253-9624 PMID: 18642666 Document Number: 617393
Objective To study the distribution of aldehyde dehydrogenase-2 (ALDH2) polymorphisms between healthy Zhuang and Han ethnic individuals in Guangxi Autonomous Region and its influence to the behaviors of alcohol consumption. Methods Polymerase chain reaction with confronting two-pair primers (PCR-CTPP) and polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) techniques were used to genotype ALDH2, respectively, and alcohol consumption was recorded in a constructed questionnaire. Results The frequencies of ALDH2 alleles (ALDH2(1)/ALDH2(2)) among Zhuang and Han ethnics were 0.511, 0.489 and 0.508, 0.492 respeetively(X-2 = 0.001, P > 0.05). The ALDH2 genotypes were verified with PCR-RFLP method. The frequencies of ALDH2(1) genotype in alcoholics (>= 3 times drinking per week) were 35.59% and 15.67% in Zhuang and Han groups respectively(X-2 = 5.800, P = 0.016). Conclusion There was no significant different distribution of ALDH2 genotype among healthy Zhuang and Han ethnic people. The genotype of ALDH2 in different ethnicity might influence individual behavior of alcohol consumption.