Hemoglobin e detection using PCR with confronting two-pair primers
Intorasoot, S.; Thongpung, R.; Tragoolpua, K.; Chottayaporn, M.
Journal of the Medical Association of Thailand 91(11): 1677-1680
2008
ISSN/ISBN: 0125-2208 PMID: 19127788 Document Number: 12872
To develop and apply the polymerase chain reaction with confronting two-pair primers (PCR-CTPP) for detection and identification of hemoglobin E (Hb E). Fifty unrelated northern Thais were included in the present study. DNA was extracted from peripheral blood mononuclear cells and targeted to amplify by PCR-CTPP. The amplified product was analyzed and compared with the reference hemoglobin electrophoresis and polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis. The results validated a completely concordant among these three methods consisting of 74%, 24%, and 2% identified as normal, heterozygous, and homozygous Hb E type, respectively. Successful Hb E genotyping by PCR-CTPP was introduced. It allows for confirming and simultaneously detection with other thalassemia mutations.
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