ALL 98 strains of Brucella OMP25 gene is conservative in China
Wang, W-jing.; Li, M.; Rui, Y-yu.
Zhonghua Yu Fang Yi Xue Za Zhi 42(2): 127-129
2008
ISSN/ISBN: 0253-9624 PMID: 18642668 Document Number: 617210
Objective To investigate the genetic polymorphism of OMP25 gene isolated from 116 Brucella strains, including 18 Brucella reference, strains and 98 Chinese field strains. Methods Chromosomal DNA of Brucella strains were analyzed by PCR, and then the product OMP25 gene was digested with Hind III and separated on 10% polypropylene agarose gel electrophoresis. OMP25 genes of different types were sequenced. Results Eight gene types ( I - VIII) existing in 116 strains were examined, among them type II and VII were only found in certain wild strains. Sequencing analysis of OMP25 revealed only 10 sites varied in all 8 types. OMP25 of 98 Chinese Brucella strains was highly homologic and characteristic of stable antigen. Conclusion High conservation characteristics helps to epidemiologically detect Brucella in China as well as to found a theoretical basis for vaccine development.