Hantavirus mucosal vaccine through different mucosal with heat-labile enterotoxin B subunit as adjuvants
Cao, S-Chun.; Li, J-Dong.; Lu, P.; Liu, F.; Wang, Q.; Liu, Q-Zhi.; Zhang, Q-Fu.; Li, C.; An, Q.; Liang, M-Fang.; Li, D-Xin.
Zhonghua Shi Yan He Lin Chuang Bing du Xue Za Zhi 22(3): 174-176
2008
ISSN/ISBN: 1003-9279 PMID: 19031694 Document Number: 616708
In this study, an inactivated Hantavirus mucosal vaccine was produced using recombinant Escherichia coli heat-labile enterotoxin B subunit (rLTB) as adjuvant. After expression and purification, the inactivated Hantavirus strain 84Fli was used for immunization of C57 BL/6 mice through the intranasal, oral and vaginal routes. Specific IgG and secretory IgA levels were detected by ELISA in serum and vaginal washing samples. Results showed that rLTB was efficiently expressed under the induction of lactose, identified by western blotting and GM-1 binding experiments. The vaccination through the intranasal, oral and vaginal routes induced IgG and secretory IgA response. Thus, inactivated Hantavirus can produce a mucosal immune response with rLTB as adjuvant through intranasal, oral and vaginal vaccination.