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The use of fluorescent liposomal immunoanalysis for the diagnostics and indication of glanders and melioidosis

Skopinskaia, S.N.; Iarkov, S.P.; Khramov, E.N.; Khrapova, N.P.; Piven', N.N.; Prokhvatilova, E.V.

Vestnik Rossiiskoi Akademii Meditsinskikh Nauk 12: 17-22

2007

ISSN/ISBN: 0869-6047
PMID: 18228662
Document Number: 614512
The authors have developed a homogenous method for the detection of the cells, surface antigen, and Ag8 of glanders and melioidosis pathogens and antibodies to them, based on the compliment-dependent lysis of Ag8-sensitized liposomes. The method is highly specific; its sensitivity to antibodies to glanders and melioidosis pathogens is 24 to 440 times higher than that of RID: its sensitivity to Ag8 is 100 ng/ml corresponding to 1.25x10-10 M, and its sensitivity to cells is 10(5) to 10(6) cells/ml).

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