Nuclear expression of S100A4 is associated with lymph node metastasis in gastric carcinoma
Zhong, X.-y.; Zhang, L.-h.; Jia, S.-q.; Shi, T.; Du, H.; Hu, Y.; Zhang, G.-g.; Lu, A.-p.; Li, J.-y.; Ji, J.-f.
Zhonghua Wei Chang Wai Ke Za Zhi 10(5): 454-457
2007
ISSN/ISBN: 1671-0274 PMID: 17851788 Document Number: 613549
To investigate the intracellular localization of S100A4 in gastric carcinoma cells and the relationship between S100A4 expression status and lymph node metastasis of gastric carcinoma. Western blotting analysis was performed to locate the expression of S100A4 protein in sub-fraction components of frozen tissues. S100A4 protein expression was also determined by immunohistochemical method in 131 samples of gastric cancer and 20 samples of matched metastatic lymph nodes. Thirty-two of 131 (24.4%) gastric carcinoma showed positive S100A4 nuclear expression and 50/131 (38.2%) carcinoma showed positive cytoplasmic expression. In 32 samples with positive S100A4 nuclear expression, 30 (93.8%) carcinomas had positive lymph node metastases. S100A4 nuclear expression level was higher in gastric carcinoma with lymph node metastasis (29.1%) than that without lymph node metastasis (7.1%) (P=0.016). Nuclear expression of S100A4 is associated with lymph node metastasis of gastric carcinoma.