The use of multilocus sequence typing (MLST) and randomly amplified polymorphic DNA (RAPD) for the differentiation between strains of Burkholderia mallei
Antonov, V.A.; Altukhova, V.V.; Savchenko, S.S.; Zamaraev, V.S.; Iliukhin, V.I.; Alekseev, V.V.
Molekuliarnaia Genetika Mikrobiologiia i Virusologiia 3: 3-9
2007
ISSN/ISBN: 0208-0613 PMID: 17891848 Document Number: 612230
Burkholderia mallei is highly pathogenic microorganism for both humans and animals. In this work, the possibility of the use of the genotyping method for differentiation between strains of B. mallei was studied. A collection of 14 isolates of B. mallei was characterized using randomly amplified polymorphic DNA (RAPD) and multilocus sequence typing (MLST). RAPD was the best method used for detecting strain differences of B. mallei. It was suggested that this method would be an increasingly useful molecular epidemiological tool.