Significance of urinary excretion of 8-hydroxy-2'-deoxyguanosine in healthy subjects and liver disease patients
Ichiba, M.; Yamada, S.; Ishii, K.; Gonda, K.; Murai, R.; Shimomura, T.; Saeki, T.; Kanbe, T.; Tanabe, Y.; Yoshida, Y.; Tsuchiya, H.; Hoshikawa, Y.; Kurimasa, A.; Kishimoto, Y.; Kawasaki, H.; Shiota, G.
Hepato-Gastroenterology 54(78): 1736-1740
2007
ISSN/ISBN: 0172-6390 PMID: 18019707 Document Number: 611981
Background/Aims: Although the importance of reactive oxygen species (ROS) in the pathogenesis of various diseases is stressed, clinical significance of the markers reflecting DNA oxidation such as 8hydroxy-2'-deoxyguanosine (8-OHdG) remains to be clarified.Methodology: To examine clinical usefulness of 8OHdG in healthy individuals in comparison with liver disease patients, urinary excretion of 8-OHdG was measured in 336 healthy individuals and 110 patients with liver disease.Results: In healthy persons, the 8-OHdG excretion was increased in an age-dependent manner. It was positively correlated with cigarettes smoked a day and negatively correlated with body mass index (BMI) (P<0.05, each). Age, smoking and BMI were independent predictors of urinary 8-OHdG excretion (P<0.01, P<0.01 and P<0.05, respectively). In liver disease, the excretion of 8-OHdG was not changed, as compared with healthy individuals. However, the liver disease patients under the age of 40 had higher values of 8-OHdG than healthy persons. In addition, the urinary excretion of 8-OHdG was higher in patients with hepatitis C virus (HCV) infection than those with hepatitis B virus (HBV) infection.Conclusions: The results of the present study suggest that measurement of urinary 8-OHdG excretion is useful in assessing DNA oxidation caused by aging, smoking, body composition and liver disease.