Peripheral arterial revascularization causing parallel increased activity of pro- and anti-inflammatory mediators
Parmar, J.H.; Aslam, M.; Standfield, N.J.
International Angiology a Journal of the International Union of Angiology 26(1): 8-11
2007
ISSN/ISBN: 0392-9590 PMID: 17353882 Document Number: 611850
Aim. The aim of this study was to investigate the interaction between the proinflammatory mediator interleukin (IL)-6 and anti-inflammatory mediator IL-10 in ischemia reperfusion injury following peripheral arterial revascularization.Methods. Three groups of patients were recruited (group 1: femoral angiography alone [n=14]; group 2: radiologically successful ilio/femoral angioplasty [n= 10]; and group 3: peripheral surgical bypass [n= 11]). Peripheral venous blood obtained for all patients at preprocedure and at I h and 24 h postprocedure. After centrifugation supernatant plasma was separated and analysed for proinflammatory cytokine IL-6 and anti-inflammatory cytokine IL-10 using enzyme linked immunosorbent assay (ELISA) technique.Results. Levels of IL-6 at 1 and 24 h were significantly higher in group 3 (P < 0.05 and P < 0.01, respectively) and at 24 h in group 2 as compared to group I (P < 0.05). Levels of IL-10 were significantly higher in group 3 at 24 h (P < 0.05). In group 2 levels of IL-10 higher than group 1, but this did not reach statistical significance (P=0.07).Conclusion. Raised levels of IL-6 at 1 and 24 h in patients undergoing operative peripheral bypass suggest early and persistent proinflammatory activity, similarly patients with successful angioplasty also demonstrated similar persistent proinflarnmatory activity of IL-6 at 24 h. Raised levels of IL-10 at 24 h represents anti-inflammatory properties of IL-10 counter acting inflammatory response.