Rapid micropropagation via axillary bud proliferation of Adhatoda vasica Nees from nodal segments
Abhyankar, G.; Reddy, V.D.
Indian Journal of Experimental Biology 45(3): 268-271
2007
ISSN/ISBN: 0019-5189 PMID: 17373372 Document Number: 610192
A protocol for rapid multiplication of Adhatoda vasica has been developed through nodal explants from field grown mature plants. The maximum number of shoots, i.e., 7.75 +/- 0.392 differentiated from split nodal halves on MS medium supplemented with BA (10.0 mg/l) during 4 weeks of culture. Maximum number of shoots formed per explant increased to ca. 30 within 6 weeks of subculture on medium containing BA (1.0 mg/l) and Kn (1.0 mg/l). The isolated shoots rooted 90% in MS medium containing IBA (0.1 mg/l) in 2 weeks. The rooted plantlets were successfully transferred to soil in glasshouse and subsequently in field. The plantlets rooted in liquid medium did not survive, but those rooted on solid medium showed more than 75% survival. hi vitro raised plants grew successfully ex vitro till flowering.