Primary culture of mammalian nephron epithelia: requirements for cell outgrowth and proliferation from defined explanted nephron segments
Horster, M.
Pflugers Archiv European Journal of Physiology 382(3): 209-215
1979
ISSN/ISBN: 0031-6768 PMID: 575410 Document Number: 141041
Nephron segments were dissected from fetal or from early postnatal rabbit kidneys (n = 86) and explanted individually into primary tissue culture. Outgrowth of epithelioid cells and proliferation in monolayers from the collecting tubule and the thick ascending limb of Henle's loop occurred regularly if a special substratum and matrix were used. Media were either supplemented with non-proteins (defined media) or with embryo extract and fetal sera (undefined media). Outgrowth and monolayer spreading were recorded by differential interference contrast microscopy. Monolayer cells resemble those seen with the same recording technique during tubule in vitro perfusion. Undefined media were essential for growth of convoluted tubule segments from embryonic nephron anlagen and outgrowth of cells from proximal straight tubules, in contrast to the distal nephron segments. The specific functional and morphological properties of cells derived from primary culture of nephron segments are under study.