Detection of gamma-hydroxybutyrate (GHB) in beverages
Liu, W.; Shen, M.; Xiang, P.; Bu, J.; Wu, H.-J.
Fa Yi Xue Za Zhi 23(2): 120-122; 129
2007
ISSN/ISBN: 1004-5619 PMID: 17619459 Document Number: 609919
To establish an analytical method for the determination of GHB in beverages using GC/MS and LC/MS/MS. After beverage samples with GHB-d6 as the internal standard were extracted with ethyl acetate, then the extracts were derivatized with N,O-bis(trimethylsilyl)trifluoroacetamide (BSTFA), at last the derivateized extracts analyzed by gas chromatography- mass spectrometry. After beverage samples with GHB-d6 as the internal standard were diluted by mobile phase then directly analyzed by LC/MS/MS. Results The limit of detection was 0.2 microg/mL and both relative standard deviations for between-day and within-day assays were < 8.54% in GC/MS. The limit of detection was 2 microg/mL and both relative standard deviations for between-day and within-day assays were <8.62% in LC/MS/MS. Conclusion These methods of qualitative and quantitative analysis were found to be sensitive, accurate, rapid and suitable for the forensic toxicology to test of GHB in real cases.