The role of transcription factors in the response of mouse lymphocytes to low-level electromagnetic and laser radiations
Khrenov, M.O.; Cherenkov, D.A.; Glushkova, O.V.; Novoselova, T.V.; Lunin, S.M.; Parfeniuk, S.B.; Lysenko, E.A.; Novoselova, E.G.; Fesenko, E.E.
Biofizika 52(5): 888-892
2007
ISSN/ISBN: 0006-3029 PMID: 17969924 Document Number: 608302
The effects of low-intensity laser radiation (LILR, 632.8 nm, 0.2 mW/cm(2)) and low-intensity electromagnetic waves (LIEW, 8.15 - 18 GHz, I MW/cm(2)) on the production of transcription factors in lymphocytes from NMR1 male mice were examined. The total level of NF-kappa B and its phosphorylated metabolite Phospho-NF-kappa B, as well as the regulatory protein I kappa B-alpha were determined in spleen lymphocytes subjected to laser or microwave radiations. The proteins were determined by immunoblotting. Laser light induced a lowering in the level of NF-kappa B I kappa B-alpha. By contrast, irradiation with electromagnetic waves resulted in a significant increase in the amount of NF-kappa B I kappa B-alpha. The phosphorylated form of NF-kappa B did not noticeably change under either of the two kinds of radiation. The results showed that electromagnetic waves activate the production of both NF-KB and the regulatory protein I kappa B-alpha and these data confirm the stress character of the response of spleen lymphocytes to low-level microwaves of the centimeter range.