Genetic polymorphisms of 10 STR (AmpFlSTR SGM Plus system) loci in Gypsy population from Poland
Michalczak, R.; Kałuzewski, B.; Berent, J.ła.
Annales Academiae Medicae Stetinensis 53(Suppl 2): 136-138
2007
ISSN/ISBN: 1427-440X PMID: 20143697 Document Number: 608144
The aim of the paper was to create a database of the allele distribution at 10 STR loci (D3S1358, vWA, D16S539, D2S1338, D8S1179, D21S11, D18S51, D19S433, TH01, FGA). The DNA were isolated from samples collected from 222 unrelated individuals of the Gypsy population from Poland. Amplification was performed using a commercial multiplex FlSTR SGM Plus kit. The amplified fragments were resolved by electrophoresis in a 5% denaturing polyacrylamid gel using tne ABI Prism 377 DNA sequencer. The expected performance of the analyzed loci for personal identification testing was estimated. All loci met the Hardy-Weinberg assumption. The combinated values of the matching probability (MP) and of the power exclusion (PE) are 3.95 x 10(-13) and 0.9999641. Statistical parameters (PIC, MP, PE) showed that the examined systems are useful for forensic medicine.