Population genetic data on the STR loci D2S1338, D19S433 and SE33 in Hungary
Egyed, B.; Csikai, M.; Füredi, S.; Padar, Z.
Journal of Forensic Sciences 50(3): 720-721
2005
ISSN/ISBN: 0022-1198 PMID: 15932119 Document Number: 590194
Blood samples of 225 unrelated individuals living in Central Hungary were collected irrespective of ethnic background in a blood bank of Budapest (1). The genomic DNA of the samples was isolated from whole blood using proteinase K digestion, organic extraction and Microcon-100 (Millipore) ultrafiltration. PCR amplification was carried out on 1-2 ng template DNA in 25 mu L reaction volume following manufacturers' instructions (AmpFeSTR Identifiler PCR amplification kit, Applied Biosystems; PowerPlex((R)) ES System, Promega). The PCR products were analyzed on AB 13 100 Genetic Analyzer. For fragment sizing and genotyping GeneScan Analysis v3.1.2 and Genotyper v2.5.2 softwares were used. Analysis of the Hardy-Weinberg equilibrium was performed by the exact test (2), as well as the observed and expected heterozygosity values were calculated using the Arlequin ver.2001 software (http://lgb.unige.ch/ariequin) (3). Allelic designation and nomenclature of the genotypes during the analyses was done according to previous recommendations (4,5). Until now no published data were available for the loci D2S1338 and D19S433 in the Hungarian population. Genotyping the population sample at the locus SE33 one sample possessed three alleles (14,16,17) showing a well-balanced three-allele pattern. This and three other samples that have carried 16.3 or 18.3 alleles at locus SE33 have been re-extracted and reanalyzed. The repeated analyses showed the same allele pattern. The sample harbouring the three-allele pattern was removed from the database, therefore the original sample size decreased from 225 to 224. The forensically informative population genetic data are demonstrated in Table 1.Access to the data: Available upon request via electronic mail from corresponding author, Balazs Egyed, at balazs.egyed@mail. orfk.b-m.hu