Semi-automated selection of DNA aptamers using magnetic particle handling
Wochner, A.; Cech, B.; Menger, M.; Erdmann, V.A.; Glökler, Jörn.
Biotechniques 43(3): 344 346 348 Passim
2007
ISSN/ISBN: 0736-6205 PMID: 17907577 Document Number: 607044
We have developed a semi-automatic selection procedure for DNA aptamers. Employing a robotic workstation for magnetic particle handling, this method allows for a fast, reproducible, and parallelized selection of DNA aptamers. The selection protocol is designed to provide and reagents, as well as vide high flexibility and versatility in terms of choice of buffers and reagents, as well as stringency of selection. Using this procedure, we have successfully isolated ligand-specific, high-affinity DNA aptamers.