Semi-automated fluorometry of total estrogens in plasma during late pregnancy
Goutte-Coussieu, C.; Sanchez, D.; Adessi, G.; Jayle, M.F.
Clinical Chemistry 26(2): 190-193
1980
ISSN/ISBN: 0009-9147 PMID: 7353263 Document Number: 154686
We describe a mechanized method for determining total estrogens in plasma during the last trimester of pregnancy. This method involves rapid enzymic hydrolysis, automatic extraction (in a tube) with cyclohexane/ethyl acetate, purification of the extracted material by anion-exchange chromatography on a disposable "mini-column" of Dowex AG1 X 2 (acetate form), and continuous-flow fluorometric quantification. Conditions of hydrolysis and extraction were studied. The mean within-day imprecision (CV) was 7% and between-day imprecision was 8.1%. Sensitivity was 75 nmol/L of plasma. Results are obtained in 4 h. The technique is quite suitable for routine determinations: 90 assays can be done by a team of three technicians in one working day.