Chromosomal damage in two X-ray irradiated cell lines: influence of cell cycle stage and irradiation temperature
Larsson, D.E.; Gustavsson, S.; Hultborn, R.; Nygren, J.; Delle, U.; Elmroth, K.
Anticancer Research 27(2): 749-753
2007
ISSN/ISBN: 0250-7005 PMID: 17465198 Document Number: 606125
The aim of this study was to investigate if irradiation with X-rays in different cell cycle phases resulted in a different response as measured with the micronucleus technique. In addition, the influence of irradiation temperature was investigated. Materials and Methods: Cells from a non-transformed human fibroblast cell line, HS2429, and a human breast cancer cell line, MCF-7, were synchronized by thymidine block and irradiated at either 2 degrees C or 37 degrees C in the G1-, S- and G2/M-phases. After cytokinesis-block by cytochalasin B, the frequency of micronuclei was deten-nined. Results: Clear dose-response relationships were found. More micronuclei were detected in fibroblast cells irradiated in G1 and S than in G2/M, while the differences were not as prominent in MCF-7 cells. The irradiation temperature had no significant influence on the formation of micronuclei in either of the cell lines. Conclusion: The formation of micronuclei varies with the cell cycle stage at the time of irradiation.