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Sodium butyrate-dependent sensitization of human colon adenocarcinoma COLO 205 cells to TNF-alpha-induced apoptosis

Pajak, B.; Orzechowski, A.

Journal of Physiology and Pharmacology An Official Journal of the Polish Physiological Society 58(Suppl 3): 163-176

2007

ISSN/ISBN: 0867-5910
PMID: 17901592
Document Number: 606054
COLO 205 colon adenocarcinoma cells are highly resistant to extrinsic apoptosis induced by immunomodulatory cytokines. One of the antiapoptotic mechanisms is the expression of cFLIP protein, which inhibits TNF-alpha-induced cell death. The use of metabolic inhibitors, such as sodium butyrate (NaBt), the potent repressor of histone deacetylase, sensitizes tumor cells to TNF-alpha-mediated apoptosis. The Western-blot analysis revealed that in COLO 205 cells the susceptibility to apoptogenic stimuli results from time-dependent reduction in cFLIP(L) protein assembled with DISC complex. At the same time, the level of transmembrane TNF-alpha receptor 1 (TNF-R1) was elevated which is consistent with the exaggerated rate of cell death. Since preincubation of COLO 205 cells with N-acetyl-L-cysteine (NAC), or sodium ascorbate (ASC) did not protect cells from combined NaBt- and TNF-alpha-induced apoptosis, we concluded that deletion of cancer cells is not evoked by oxidative stress. Our results suggest that the combination of TNF-alpha with NaBt targets antiapoptotic protein(s) and may provide efficient and non-toxic treatment of colon cancer.

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Xi, L.; Wu, M-Fu.; Wu, J-Hong.; Li, F-Jun.; Lu, Y-Ping.; Zhou, J-Feng.; Ma, D. 2005: Changes in telomerase activity decreases in MCF-7 cells during apoptosis induced by sodium butyrate Zhonghua Zhong Liu Za Zhi 27(1): 9-12
Shitikova, Z.V.; Aksenov, N.D.; Pospelov, V.A.; Pospelov, T.V. 2011: Cell senescence induced by histone deacetylase inhibitor sodium butyrate in rodent transformed cells resistant to apoptosis Tsitologiia 53(3): 277-284
Dexter, D.L.; Crabtree, G.W.; Stoeckler, J.D.; Savarese, T.M.; Ghoda, L.Y.; Rogler-Brown, T.L.; Parks, R.E.; Calabresi, P. 1981: N,N-dimethylformamide and sodium butyrate modulation of the activities of purine-metabolizing enzymes in cultured human colon carcinoma cells Cancer Research 41(3): 808-812
Tsao, D.; Morita, A.; Bella, A.; Luu, P.; Kim, Y.S. 1982: Differential effects of sodium butyrate, dimethyl sulfoxide, and retinoic acid on membrane-associated antigen, enzymes, and glycoproteins of human rectal adenocarcinoma cells Cancer Research 42(3): 1052-1058
Gabius, S.; Yamazaki, N.; Hanewacker, W.; Gabius, H.J. 1990: Regulation of distribution, amount and ligand affinity of sugar receptors in human colon carcinoma cells by treatment with sodium butyrate, retinoic acid and phorbol ester Anticancer Research 10(4): 1005-1012
Wang, M.-J.; Liu, Y.-X.; Zhang, J.-C.; Liu, S.-L.; Zheng, D.-X. 2005: Mechanisms of sensitization by 8-chloro-adenosine of human tumor cells to TRAIL-induced apoptosis Zhonghua Zhong Liu Za Zhi 27(10): 586-590
Litvak, D.A.; Hwang, K.O.; Evers, B.M.; Townsend, C.M. 2000: Induction of apoptosis in human gastric cancer by sodium butyrate Anticancer Research 20(2a): 779-784
Cook, J.R.; Schwartz, C.E.; Fausel, E.D.; Chiu, J.F. 1985: Effect of sodium butyrate on alpha-fetoprotein gene expression in rat hepatoma cells in vitro Cancer Research 45(7): 3215-3219
Zhang, M-sheng.; Zhou, Y-feng.; Zhang, W-jie.; Zhang, X-lian.; Pan, Q.; Ji, X-mei.; Luo, Z-guo.; Wu, J-ping. 2006: Apoptosis induced by short hairpin RNA-mediated STAT6 gene silencing in human colon cancer cells Chinese Medical Journal 119(10): 801-808
Ryu, J.C.; Seo, Y.R.; Smith, M.L.; Han, S.S. 2001: The effect of methyl methanesulfonate (MMS)-induced excision repair on p53-dependent apoptosis in human lymphoid cells Research Communications in Molecular Pathology and Pharmacology 109(1-2): 35-51
Su, C-Cheng.; Chen, G-Wei.; Tan, T-Wei.; Lin, J-Gung.; Chung, J-Gung. 2006: Crude extract of garlic induced caspase-3 gene expression leading to apoptosis in human colon cancer cells In Vivo 20(1): 85-90
Glazyrin, A.L.; Chinni, S.; Alhasan, S.; Adsay, V.N.; Vaitkevicius, V.K.; Sarkar, F.H. 2002: Molecular mechanism(s) of actinomycin-D induced sensitization of pancreatic cancer cells to CD95 mediated apoptosis International Journal of Oncology 20(1): 201-205
Zhang, X.-F.; Li, B.-X.; Ren, R. 2006: Effect of ceramide on apoptosis of human colon cancer HT-29 cells Wei Sheng Yan Jiu 35(5): 537-540
Unkles, S.; McDevitt, T.; Voy, C.; Kinghorn, J.R.; Cramb, G.; Lamb, J.F. 1988: The effect of growth in monensin or low potassium on internal sodium, alpha sub-unit mRNA and sodium pump density in human cultured cells Progress in Clinical and Biological Research 268b: 157-162
Lührs, H.; Kudlich, T.; Neumann, M.; Schauber, Jürgen.; Melcher, R.; Gostner, A.; Scheppach, W.; Menzel, T.P. 2002: Butyrate-enhanced TNFalpha-induced apoptosis is associated with inhibition of NF-kappaB Anticancer Research 22(3): 1561-1568
Domoto, D.T.; Askenase, P.W. 1980: H-2-dependent cell-mediated immunity in vivo: delayed-type hypersensitivity and contact sensitization induced by fluorescein isothiocyanate-conjugated cells Journal of Immunology 125(5): 2161-2166
Lockshin, A.; Giovanella, B.C.; Mendoza, J.T.; Warneke, C.; Stehlin, J.S. 1985: Selective cytotoxicity of 5-hydroxyuridine for human colon adenocarcinoma cells Cancer Treatment Reports 69(7-8): 845-850
Tsuchiya, T.; Tsuno, N.H.; Asakage, M.; Yamada, J.; Yoneyama, S.; Okaji, Y.; Sasaki, S.; Kitayama, J.; Osada, T.; Takahashi, K.; Nagawa, H. 2008: Apoptosis induction by p38 MAPK inhibitor in human colon cancer cells Hepato-Gastroenterology 55(84): 930-935
Sheng, H.; Shao, J.; Morrow, J.D.; Beauchamp, R.D.; DuBois, R.N. 1998: Modulation of apoptosis and Bcl-2 expression by prostaglandin E2 in human colon cancer cells Cancer Research 58(2): 362-366
Boudny, V.; Nakano, S. 2003: Src tyrosine kinase but not activated Ras augments sensitivity to taxanes through apoptosis in human adenocarcinoma cells Anticancer Research 23(1a): 7-12