Slow Ca2'-binding Mg2+- EDTA buffer for intracellular perfusion
Berestovskiĭ, G.N.; Kataev, A.A.; Grinevich, A.A.
Biofizika 52(3): 466-468
2007
ISSN/ISBN: 0006-3029 PMID: 17633535 Document Number: 606046
In studies of currents in perfused cells or their membrane fragments containing potential-dependent Ca channels and Ca2+-activated channels, a buffer slowly binding Ca2+ appears useful in some cases. A buffer with EDTA in excess of Mg2+ has been proposed, and its kinetic characteristics have been calculated. It has been shown that this buffer, depending on its component proportions, may provide Ca2+ binding with a characteristic time of up to tens of milliseconds. For comparison, in the cytoplasm, this time does not exceed 1 ms even with a large calcium signal, as follows from calculations.