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Isolation and identification of Japanese encephalitis virus in Liaoning Province

Wang, J-wen.; Fu, S-hong.; Wang, H-yu.; Mao, X-yan.; Liu, W-bin.; He, Y.; Sun, X-hong.; Cai, Z-lin.; Wu, L-ping.; Zhao, X-fang.; Han, R-hong.; Jing, Y.; Liang, G-dong.

Zhonghua Shi Yan He Lin Chuang Bing du Xue Za Zhi 20(1): 61-65

2006

ISSN/ISBN: 1003-9279
PMID: 16642222
Document Number: 604697
A study was conducted to investigate arboviruses carried by mosquitoes in Liaoning Province, China. 4927 mosquitoes were collected from Liaoning Province in July 2002. Virus strains were isolated by inoculation into BHK-21, C6/36 and Vero cells. The newly isolated strains were identified by serological (ELISA and IFA) and molecular methods (real-time polymerase chain reaction (PCR)). Two strains were isolated from mosquitoes causing cytopathogenic effect on cells and were fatal to suckling mice. Serological tests showed that both strains were positive for Japanese encephalitis virus (JEV) antibody. The PrM and E genes were cloned and sequenced. The phylogenetic analysis showed that the new isolates belonged to genotype I, JEV. Sequence analysis showed that the homology of nucleotide sequences and amino acid (AA) sequences between the 2 strains was 100%. Compared with the nucleotide sequences between the 2 strains and the standard JEV vaccine strain SA14-14-2, the difference was up to 4.11%, and the difference of AA was 0.6%.

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Feng, G.X. 1984: Surveillance of Japanese encephalitis B in Shenyang, Liaoning Province 1975-1982 Zhonghua Liu Xing Bing Xue Za Zhi 5(3): 129-133
Doi, R.; Oya, A.; Shirasaka, A.; Yabe, S.; Sasa, M. 1983: Studies on Japanese encephalitis virus infection of reptiles. II. Role of lizards on hibernation of Japanese encephalitis virus Japanese Journal of Experimental Medicine 53(2): 125-134
Zhang, J.-K.; Lin, S.-H.; Zhou, X.-Y.; Chen, D.-L.; Fu, S.-H.; Liu, X.-C.; Yuan, W.; Cao, Y.-O.; Wang, H.-Y.; Li, W.; Hu, M.; Liang, G.-D. 2012: Japanese encephalitis virus with genotype i is predominant in Sichuan Province Zhonghua Shi Yan he Lin Chuang Bing du Xue Za Zhi 26(4): 241-245
Zhang, H.L. 1990: The natural infection rate of mosquitoes by Japanese encephalitis B virus in Yunnan Province Zhonghua Yu Fang Yi Xue Za Zhi 24(5): 265-267
Xie, R.-h.; Zhang, X.-f.; Zhu, H.-p.; Xu, F.; Yao, P.-p.; Cheng, Y.-k.; Zhang, Y.-j.; Zhu, Z.-y. 2009: The distributions of mosquito vectors carrying Japanese encephalitis virus in Zhejiang province Zhonghua Yu Fang Yi Xue Za Zhi 43(4): 282-286
Yasri, S.; Wiwanitkit, V. 2014: Finding of B cell epitope within polyprotein of Japanese encephalitis virus: a clue for development of new Japanese encephalitis vaccine Arquivos de Neuro-Psiquiatria 72(6): 478
Gupta, A.K.; Lad, V.J.; Koshy, A.A. 2008: Survival of mice immunized with monoclonal antibodies against glycoprotein e of Japanese encephalitis virus before or after infection with Japanese encephalitis, West Nile, and Dengue viruses Acta Virologica 52(4): 219-224
Deriabin, P.G.; Sveshnikova, N.A.; Rodova, M.A.; Mikhaĭlova, G.R.; Loginova, N.V. 1982: Karyological characteristics of L cells and the identification with them, of the antigen of persisting Japanese encephalitis virus Voprosy Virusologii 1: 48-53
Jiang, H.-Y.; Li, M.-H.; Yang, X.-B.; Li, P.-L.; Zhang, J.; Meng, F.-X.; Guo, Y.-H.; Mei, L.-Z.; Fu, S.-H.; He, Y.-Y.; Wang, H.-Y.; Wang, Z.-J.; Yang, W.-Z.; Wang, Z.-Y.; Liang, G.-D. 2010: Molecular characteristics of the full-length genome of two new Japanese encephalitis virus isolated from mosquitoes in Hubei province Zhonghua Yu Fang Yi Xue Za Zhi 44(5): 438-443
Hötzel, I.; Bastos, E.d.S.; Ravazzolo, A.P.; Moojen, V. 1993: Caprine arthritis-encephalitis virus: isolation and identification in Rio Grande do Sul, Brazil Brazilian Journal of Medical and Biological Research 26(11): 1175-1179
Kedarnath, N.; Prasad, S.R.; Dandawate, C.N.; Koshy, A.A.; George, S.; Ghosh, S.N. 1984: Isolation of Japanese encephalitis & West Nile viruses from peripheral blood of encephalitis patients Indian Journal of Medical Research 79: 1-7
George, S.; Prasad, S.R.; Rao, J.A.; Yergolkar, P.N.; Setty, C.V. 1987: Isolation of Japanese encephalitis and West Nile viruses from fatal cases of encephalitis in Kolar district of Karnataka Indian Journal of Medical Research 86: 131-134
Qu, Y.-g.; Yang, G.-q.; Zou, Y.; Yan, G.-q.; Chen, H.-x.; Zhang, Y.-z. 2006: Isolation and characterization of Hantavirus carried by rodents in Huludao, Liaoning province Zhonghua Liu Xing Bing Xue Za Zhi 27(6): 513-517
Wang, Y.; Ma, Y.; Hao, S.; Xu, X.; Han, Y.; Yao, W.; Zhao, Z. 2016: SH and HN Protein Genetic Characterization Analysis of Mumps Virus Isolated in Liaoning Province from 2008 to 2014 Bing du Xue Bao 32(2): 179-184
Tang, X-yan.; Zhang, Y-ping.; Xu, B-li.; Li, X-le. 2010: The prevalence characteristic and prevention strategy of Japanese B Encephalitis in Henan province Zhonghua Yu Fang Yi Xue Za Zhi 44(4): 329-333
Haishi, S.; Imai, H.; Hirai, K.; Igarashi, A.; Kato, S. 1989: Expression of envelope glycoprotein (E) of Japanese encephalitis virus by recombinant vaccinia virus Acta Virologica 33(6): 497-503
Kelkar, S.D.; Gogate, S.S. 1987: Macrophage-virus interaction during Con A-induced protection against Japanese encephalitis virus in infant mice Acta Virologica 31(2): 103-108
Thenmozhi, V.; Paramasivan, R.; Samuel, P.Philip.; Kamaraj, T.; Balaji, T.; Dhananjeyan, K.J.; Venkatasubramani, K.; Tyagi, B.K. 2013: Dual infection in human by Japanese encephalitis virus & chikungunya virus in Alappuzha district, Kerala, India Indian Journal of Medical Research 138(3): 362-363
Tang, X.-Y.; Ma, H.-X.; Xu, C.; Shang, S.-Y.; Kang, K.; Huang, X.-Y.; Yin, Z.-D.; Wang, H.-Y. 2011: Surveillance of healthy people's Japanese encephalitis neutralizing antibody in some areas of Henan Province Zhonghua Shi Yan he Lin Chuang Bing du Xue Za Zhi 25(6): 401-404
An, Z.-J.; Ma, Y.-J.; Bo, F.; Huang, H.; Zhang, S.-T. 2007: Analysis on surveillance data regarding the level of Japanese encephalitis antibody in Heilongjiang. province, 2006 Zhonghua Liu Xing Bing Xue Za Zhi 28(12): 1186-1189