1H NMR study of the complexation of the quinolone antibiotic norfloxacin with DNA
Evstigneev, M.P.; Baranovskiĭ, S.F.; Rybakova, K.A.; Davis, D.B.
Molekuliarnaia Biologiia 40(5): 894-899
2006
ISSN/ISBN: 0026-8984 PMID: 17086991 Document Number: 600575
Complexation of antibiotic norfloxacin (NOR) with DNA fragments 5'-d(TpGpCpA) and 5'-d(CpGpCpG) has been studied in aqueous solution by 1H NMR spectroscopy (500 MHz). Equilibrium parameters of the complexation with single-stranded and duplex forms of DNA oligomer--equilibrium constants, enthalpy and entropy--have been obtained for the first time. Based on the analysis of the complexation parameters as well as induced chemical shifts of the antibiotic protons within different complexes, it was found that NOR binds with the tetramer duplexes mainly by intercalation. The complexation with the single-stranded form may occur either by intercalation and external binding. The site of preferential binding of the antibiotic with DNA duplex is GC site.