Expression of Nm23 in gliomas and its effect on migration and invasion in vitro
Jung, S.; Paek, Y-Woong.; Moon, K-Sub.; Wee, S-Chan.; Ryu, H-Hwa.; Jeong, Y-Il.; Sun, H-Suk.; Jin, Y-Hao.; Kim, K-Keun.; Ahn, K-Youn.
Anticancer Research 26(1a): 249-258
2006
ISSN/ISBN: 0250-7005 PMID: 16475705 Document Number: 597810
Background: Despite the well-documented importance of Nm23 in the control of metastasis, there is currently a paucity of data regarding the role of this gene family in the control of glioma invasion. Materials and Methods: Nm23-H1 expression in gliomas was assessed via immunohistochemistry, Western blot, RT-PCR and Northern blot analyses. The migration and invasion ability were also investigated in primary glioma culture cells, human glioma cell lines and nm23-H1 transfectant, using an in vitro brain slice invasion model and a simple scratch technique. Results: Although no significant correlations were detected between nm23-H1 expression and pathological grade, the endogenous nm23-H1 expression in gliomas was found to be inversely correlated with their migratory abilities. Additionally, the nm23-H1 transfectant resulted in a reduction of approximately 45% of the migratory ability and suppressed the invasiveness of the parental cell line. Conclusion: Our overall findings suggest that nm23-H1 may play an important role in the suppression of glioma invasion and migration.