Study on determination of entrapment efficiency of sinomenine liposomes
Zhou, L.-l.; Wang, Y.; Liu, Q.-f.; Ling, J.-j.
Zhongguo Zhong Yao Za Zhi 31(9): 731-734
2006
ISSN/ISBN: 1001-5302 PMID: 17048678 Document Number: 597262
To establish an HPLC method for the determination of entrapment efficiency of sinomenine liposomes. The liposomes and dissociated drugs were separated by sephadex filtration, mini-column centrifugation and dialysis. The methodology study and the optimization of determining condition were carried out at the same time. Sephadex filtration could effectively separate the sinomenine liposomes from dissociated sinomenine. The column recovery was 98.8%, the average entrapment efficiency of three tests was64.9%, RSD 2.67%. The method was simple, exact, and had a good reappearance. It can be used to examine the entrapment efficiency of sinomenine liposomes.