Role of mitochondria in reglulation of endothelial cell hyperpolarization to acetylcholine
Bondarenko, O.I.; Sahach, V.F.
Fiziolohichnyi Zhurnal 52(5): 6-11
2006
ISSN/ISBN: 2522-9028 PMID: 17176833 Document Number: 597050
We investigated the regulation of sustained endothelial hyperpolarization to acetylcholine in the rat aortic endothelial cells by mitochondria. Protonofore CCCP and rotenone, an electron transport chain complex I inhibitor, agents that cause mitochondria depolarization, inhibited the sustained hyperpolarization of endothelial cells. This effect was unlikely to be mediated by the free radicals since hydrogen peroxide was shown to hyperpolarize endothelial cells. It is concluded that mitochondrial Ca2+ uptake is essential in prolongation of endothelial hyperpolarization to acetylcholine.