Effect of ketogenic diet on hippocampus mossy fiber sprouting and GluR5 expression in kainic acid induced rat model
Xu, X-ping.; Sun, R-peng.; Jin, R-feng.
Chinese Medical Journal 119(22): 1925-1929
2006
ISSN/ISBN: 0366-6999 PMID: 17134593 Document Number: 596898
A study was conducted to investigate the possible antiepileptic mechanism of ketogenic diet (KD) in terms of synaptic reorganization and glutamate receptor 5 (GluR5) expression. Male Sprague-Dawley rats were divided into 4 groups: kainic acid (KA) + normal diet (ND), KA + KD, saline (NS) + ND, and NS + KD. On day 28 after birth, the rats of the experimental groups received KA (10 mg/kg, i. h.) and the saline control groups received an equivalent volume of saline. During the 8-week dietary treatment, spontaneous recurrent seizure (SRS) was recorded by observing each KA-induced animal for one hour everyday. SRS frequency per rat was significantly less in the KD-fed animals (1.40+or-1.03) than in the controls (7.36+or-3.75, t=5.024, P<0.05). In KD-fed (KA + KD and NS + KD groups) rats, beta -hydroxybutyrate rose to the level (4.51+or-2.32) mmol/litre, significantly higher than that in standard diet-fed (KA + ND and NS + ND groups) rats (0.11+or-0.03) mmol/litre, (t=6.83, P<0.05). The A of Timm granules in the inner molecular layer (IML) of the dentate gyrus in the KA-induced groups (KA + KD and KA + ND) was respectively higher than that in the saline control groups (NS + KD and NS + ND) (P<0.01). The results of Western blot indicated that GluR5 expression in KA + KD rats was significantly higher than that in KA + ND rats, although there was no significant difference in GluR5 mRNA between the two groups.