A method based on image processing and analyzing technology for estimating the activity of mesenchymal stem cells
Xie, L.; Wu, J.; Li, D.; Lin, J.; Wang, T.; Zheng, C.
Sheng Wu Yi Xue Gong Cheng Xue Za Zhi 23(2): 279-281
2006
ISSN/ISBN: 1001-5515 PMID: 16706347 Document Number: 595968
Cell culture is one of the usual methods for studying living cell and tissue, the method presented in this paper is based on image processing and analyzing technology for activity estimation of mesenchymal stem cells (MSCs). The existing activity estimation methods are costly, complex and invasive. In this method, thresholding is used to preprocess image and to separate out the growth hallow. Then the area is calculated by counting the pixels of the growth hallow. The changes of the activity estimated by this method are similar to those by corresponding cellular experiments. Compared with the existing methods in biology, medicine or medical cellular science, this method is easier, faster, cost-effective and non-invasive. The proposed method has been proved to be efficient by primary experiments of MSCs.